Day 9. Gene mapping and cloning

Well, I already thought that genetics seems easier, but this subject is tough for me, hard to capture.

Trying to determine where the gene is located and how far apart 2 genes are from one another.

• crossing over (in prophase I of meiosis)
• linked genes - the probability of recombination <>
• syntenic - genes on the same chromosome
• recombination frequency
• LOD scores
• linkage disequilibrium
• polymorhic markers

Gene cloning:

1. find linkage to a known markers;
2. identify protein coding regions (computers - promoter areas, exon-intron boundaries, starting codones, CG islands)
3. compare DNA sequences of these genes in affected and unaffected individuals.