Tuesday, November 13, 2007

Day 9. Gene mapping and cloning

Well, I already thought that genetics seems easier, but this subject is tough for me, hard to capture.

Trying to determine where the gene is located and how far apart 2 genes are from one another.
  • crossing over (in prophase I of meiosis)
  • linked genes - the probability of recombination <>
  • syntenic - genes on the same chromosome
  • recombination frequency
  • LOD scores
  • linkage disequilibrium
  • polymorhic markers

Gene cloning:

  1. find linkage to a known markers;
  2. identify protein coding regions (computers - promoter areas, exon-intron boundaries, starting codones, CG islands)
  3. compare DNA sequences of these genes in affected and unaffected individuals.

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